nebuilder hifi dna assembly cloning kit (New England Biolabs)
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New England Biolabs
nebuilder hifi dna assembly cloning kit
Nebuilder Hifi Dna Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuilder+hifi+dna+assembly+cloning+kit/NEBuilder+HiFi+DNA+Assembly+Cloning+Kit/pm42901160-740-289-295
Average 99 stars, based on 3251 article reviews
Nebuilder Hifi Dna Assembly Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuilder+hifi+dna+assembly+cloning+kit/NEBuilder+HiFi+DNA+Assembly+Cloning+Kit/pm42901160-740-289-295
Average 99 stars, based on 3251 article reviews
nebuilder hifi dna assembly cloning kit - by Bioz Stars,
2026-11
99/100 stars
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Construct:Article Title: Toward transgenic multicellular parasites: CRISPR-Cas12a germline editing of Schistosoma mansoni eggs as key to success Article Snippet: .. The entire construct pJC53.2_SmUbi-EGFP-SmUbi was assembled using the Article Title: New genetic tools in Finegoldia magna identify a conserved adhesin required for the formation of stress-tolerant aggregates Article Snippet: Upstream and downstream regions (each 2 kb in size) flanking the tuf insertion site were each amplified from genomic DNA of the corresponding F. magna strain, and PCR products were purified via the Monarch PCR & DNA Cleanup Kit (New England Biolabs). .. Next, upstream- ermB -downstream constructs were assembled via the Article Title: microRNAs control temporal patterning of the vertebrate nervous system Article Snippet: .. All plasmid constructs generated in this study were assembled using the Molecular Cloning:Article Title: Toward transgenic multicellular parasites: CRISPR-Cas12a germline editing of Schistosoma mansoni eggs as key to success Article Snippet: .. The entire construct pJC53.2_SmUbi-EGFP-SmUbi was assembled using the Article Title: Quantitative comparison of PI(3,5)P 2 biosensors reveals SnxA is the most sensitive and unbiased Article Snippet: .. Plasmids were cloned using PCR and the Cloning:Article Title: Toward transgenic multicellular parasites: CRISPR-Cas12a germline editing of Schistosoma mansoni eggs as key to success Article Snippet: .. The entire construct pJC53.2_SmUbi-EGFP-SmUbi was assembled using the Article Title: H2A.Z-H2B.V nucleosomes form a specialised scaffold for transcription initiation in Trypanosoma brucei Article Snippet: .. New plasmids made for this study were prepared by cloning a gBlock Gene Fragment (Integrated DNA Technologies) into a plasmid backbone using the Article Title: The RNA helicase KREH2 directly binds the RESC1-RESC2 complex in trypanosomatid mitochondrial RNA editing Article Snippet: A synthetic gene fragment (GenScript) containing the K564E/R566E/K569E/R570E mutation was amplified with primers containing AvrII and BsrGI sites (Supplementary Table S4). .. After digestion, the gene fragment was ligated into the vector via Gibson Assembly ( Article Title: New genetic tools in Finegoldia magna identify a conserved adhesin required for the formation of stress-tolerant aggregates Article Snippet: Upstream and downstream regions (each 2 kb in size) flanking the tuf insertion site were each amplified from genomic DNA of the corresponding F. magna strain, and PCR products were purified via the Monarch PCR & DNA Cleanup Kit (New England Biolabs). .. Next, upstream- ermB -downstream constructs were assembled via the Article Title: The autosomal deubiquitylase Trabid drives sex-divergent neurodegeneration in Drosophila Article Snippet: pAC5 plasmid backbone was digested NotI-HF and XbaI Communications Biology Article in Press restriction enzymes (NEB, UK). .. The PCR fragments and digested backbones were assembled by Article Title: microRNAs control temporal patterning of the vertebrate nervous system Article Snippet: .. All plasmid constructs generated in this study were assembled using the Article Title: Quantitative comparison of PI(3,5)P 2 biosensors reveals SnxA is the most sensitive and unbiased Article Snippet: .. Plasmids were cloned using PCR and the Plasmid Preparation:Article Title: H2A.Z-H2B.V nucleosomes form a specialised scaffold for transcription initiation in Trypanosoma brucei Article Snippet: .. New plasmids made for this study were prepared by cloning a gBlock Gene Fragment (Integrated DNA Technologies) into a plasmid backbone using the Article Title: The RNA helicase KREH2 directly binds the RESC1-RESC2 complex in trypanosomatid mitochondrial RNA editing Article Snippet: A synthetic gene fragment (GenScript) containing the K564E/R566E/K569E/R570E mutation was amplified with primers containing AvrII and BsrGI sites (Supplementary Table S4). .. After digestion, the gene fragment was ligated into the vector via Gibson Assembly ( Article Title: microRNAs control temporal patterning of the vertebrate nervous system Article Snippet: .. All plasmid constructs generated in this study were assembled using the other:Article Title: Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-MAEA Proteintech Cat# 28363-1-AP; RRID: AB_2881122 rabbit anti-TWA1 Novus Biologicals Cat# NBP1-32596; RRID: AB_2274921 mouse anti-Muskelin Santa Cruz Cat# sc-398956; RRID: AB_2737249 mouse anti-RanBP9 Santa Cruz Cat# sc-271727; RRID: AB_10710383 mouse anti-RanBP10 Proteintech Cat# 21107-1-AP; RRID: AB_10732603 mouse anti-ARMC8 Santa Cruz Cat# sc-365307; RRID: AB_10850172 rabbit anti-WDR26 Bethyl Laboratories Cat# A302-244A; RRID: AB_1730873 rabbit α-YPEL5 Proteintech Cat# 11730-1-AP; RRID: AB_2217465 mouse α-UBE2H Santa Cruz Cat# sc-100620; RRID: AB_ 2210469 rabbit α-AAMP Abcam Cat# ab178691; RRID: AB_3753450 rabbit α-ISG20L2 Proteintech Cat# 24639-1-AP; RRID: AB_2879649 rabbit α-HMGCS1 Proteintech Cat# 17643-1-AP; RRID: AB_2248359 rabbit α-uL16/RPL10 Proteintech Cat# 17013-1-AP; RRID: AB_2181278 rabbit α-uL14/RPL23 Abcam Cat# ab264369; RRID: AB_3753449 mouse α-eS6/RPS6 Cell Signaling Technology Cat# 2317; RRID: AB_2238583 mouse anti-Vinculin Merck Cat# V9131; RRID: AB_477629 mouse anti-β-actin Merck Cat# A2228; RRID: AB_476697 mouse anti-FLAG (M2) Merck Cat# F1804; RRID: AB_262044 rabbit α-HA (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 rabbit α-GFP Abcam Cat# ab290; RRID: AB_303395 mouse α-GFP Roche Cat# 11814460001; RRID: AB_390913 rabbit α-Phospho-p70 S6 Kinase (Thr389) Cell Signaling Technology Cat# 9205; RRID: AB_330944 HRP-conjugated donkey anti-mouse IgG Jackson ImmunoResearch Cat# 715-035-150; RRID: AB_2340770 HRP-conjugated donkey anti-rabbit IgG Jackson ImmunoResearch Cat# 711-035-152; RRID: AB_10015282 Chemicals, peptides, and recombinant proteins Bortezomib Cambridge Bioscience Cat# CAY10008822 TAK-243 Cambridge Bioscience Cat# HY-100487 MLN4924 Cambridge Bioscience Cat# CAY15217 Bafilomycin A1 ThermoFisher Scientific Cat# J61835.MCR Erastin Cambridge Bioscience Cat# CAY17754 Torin-1 Stratech Scientific Cat# A8312-APE Buthionine sulfoximine Merck Cat# 19176 Tunicamycin Cambridge Bioscience Cat# T104 Human TNF-α ThermoFisher Scientific Cat# PHC3015 TMTpro 16plex label set ThermoFisher Scientific Cat# A44520, batch XD347719 TMTpro-134C and TMTpro-135N labels ThermoFisher Scientific Cat# A52046, batch XD3214389 Critical commercial assays PolyJet In Vitro DNA Transfection Reagent SignaGen Cat# SL100688 Gentra Puregene Cell Kit QIAGEN Cat# 158767 Polymerase Chain Reaction:Article Title: The autosomal deubiquitylase Trabid drives sex-divergent neurodegeneration in Drosophila Article Snippet: pAC5 plasmid backbone was digested NotI-HF and XbaI Communications Biology Article in Press restriction enzymes (NEB, UK). .. The PCR fragments and digested backbones were assembled by Article Title: Quantitative comparison of PI(3,5)P 2 biosensors reveals SnxA is the most sensitive and unbiased Article Snippet: .. Plasmids were cloned using PCR and the Generated:Article Title: microRNAs control temporal patterning of the vertebrate nervous system Article Snippet: .. All plasmid constructs generated in this study were assembled using the Clone Assay:Article Title: Quantitative comparison of PI(3,5)P 2 biosensors reveals SnxA is the most sensitive and unbiased Article Snippet: .. Plasmids were cloned using PCR and the Restriction Assay:Article Title: Quantitative comparison of PI(3,5)P 2 biosensors reveals SnxA is the most sensitive and unbiased Article Snippet: .. Plasmids were cloned using PCR and the Ligation:Article Title: Quantitative comparison of PI(3,5)P 2 biosensors reveals SnxA is the most sensitive and unbiased Article Snippet: .. Plasmids were cloned using PCR and the |